Home Biology Biotechnology Principles and Processes Previous Year Questions Select the correct statement from the follow…
Biology Biotechnology Principles and Processes Previous Year Questions Single Correct MCQ
Published on: August 21, 2026

Select the correct statement from the following:

A
The polymerase enzyme joins the gene of interest and the vector DNA
B
Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzymes of optimum conditions
C
PCR is used for isolation and separation of gene of interest
D
Gel electrophoresis is used for amplification of a DNA segment

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The correct answer is:
B

Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzyme, at the optimal conditions for that specific enzyme. Agarose gel electrophoresis is employed to check the progression of a restriction enzyme digestion.
DNA ligase joins the gene of interest and the vector DNA. Gel electrophoresis is used for isolation and separation of gene of interest. PCR is used for the amplification of a DNA segment.

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